[ELK Biotechnology 한국공식대리점] Human IL1b(Interleukin 1 Beta) ELISA Kit
어스바이오는 ELK Biotechnology 한국 공식 대리점으로서 모든 제품을 전문적으로 취급 및 공급하고 있습니다.
ELK Biotechnology의 ELISA Kit을 소개드립니다.
[Human IL1b(Interleukin 1 Beta) ELISA Kit]

Product Information:
|
| Product name: |
Human IL1b(Interleukin 1 Beta) ELISA Kit |
| Reactivity: |
Human |
| Alternative Names: |
IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin |
| Assay Type: |
Sandwich |
| Sensitivity: |
5.8 pg/mL |
| Standard: |
1000 pg/mL |
| Detection Range: |
15.63-1000 pg/mL |
| Sample Type: |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: |
3.5h |
| Research Area: |
Cytokine;Tumor immunity;Infection immunity; |
| Uniprot ID: |
P01584 |
| Test principle: |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1b in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard curve:
| Concentration (pg/mL) |
OD |
Corrected OD |
| 1000.00 |
2.207 |
2.125 |
| 500.00 |
1.613 |
1.531 |
| 250.00 |
1.115 |
1.033 |
| 125.00 |
0.757 |
0.675 |
| 62.50 |
0.421 |
0.339 |
| 31.25 |
0.297 |
0.215 |
| 15.63 |
0.195 |
0.113 |
| 0.00 |
0.082 |
0.000 |
어스바이오(USBIO)는 ELK Biotechnology 한국 공식 대리점입니다.
해당 제품에 대한 문의나 ELK Biotechnology 제품의 견적 또는 문의사항이 있으시면 아래로 연락주시기 바랍니다.
Tel : 02-862-2816 / email : bio@usbio.co.kr
